Statewide trends and factors associated with genetic testing for hereditary cancer risk in Arkansas 2013–2018

Background Early identification of hereditary cancer risk would save lives, but genetic testing (GT) has been inadequate. We assessed i) trends for hereditary breast and ovarian cancer (HBOC), Lynch syndrome, and other GT and ii) factors associated with receipt of GT. Methods We used data from the Arkansas All-Payer Claims Database from January 2013 through June 2018 (commercial, Medicaid), December 2017 (state employee), or December 2016 (Medicare) and identified enrollees with ≥1 month of enrollment. Using Current Procedural Terminology (CPT-4) codes, rates for GT were calculated per 100,000 person-quarters and time series regressions estimated. Second, GT and covariate information for enrollees with 24 months of continuous enrollment were used to estimate separate logistic regression models for each GT category. Results Among 2,520,575 unique enrollees, HBOC testing rates were 2.2 (Medicaid), 22.0 (commercial), 40.4 (state employee), and 13.1(Medicare) per 100,000 person-quarters and increased linearly across all plans. Older age (OR=1.24; 95%CI 1.20 – 1.28), female sex (OR=18.91; 95%CI 13.01 – 28.86), higher comorbidity burden (OR=1.08; 95%CI 1.05 – 1.12), mental disorders (OR=1.53; 95%CI 1.15 – 2.00), and state employee coverage (OR=1.65; 95%CI 1.37 – 1.97) were positively associated with HBOC testing. Less than 1 of 10,000 enrollees received Lynch syndrome testing, while < 5 of 10,000 received HBOC testing. Conclusion GT rates for hereditary cancer syndromes have increased in Arkansas but remain low. Receipt of GT was explained with high discrimination by sex and plan type. Impact Expansion of GT for hereditary cancer risk in Arkansas is needed to identify high-risk individuals who could benefit from risk-reduction strategies. Supplementary Information The online version contains supplementary material available at 10.1186/s13053-022-00226-0.


Introduction
Screening individuals unaffected by cancer for hereditary cancer syndromes (HCS) provides opportunities to reduce cancer risk by offering preventive strategies to those with elevated risk to mitigate or even eliminate cancer development. Significant advances in our understanding of the genes that underlie the most common HCS, hereditary breast and ovarian cancer (HBOC) and Lynch syndrome, have been made since the cloning  20:19 of BRCA1 in 1994, yet no more than 1%-10% of individuals at risk for these syndromes have been identified [1][2][3]. The landscape for genetic testing for HCS has evolved rapidly in the past decade [4]. Awareness of hereditary cancer risk has increased for the public and health care providers because of education efforts and disclosures of mutation status by celebrities such as Angelina Jolie [5]. Simultaneously, multiple companies now use next-generation sequencing platforms that allow for faster testing of large panels of genes at everdecreasing cost [6,7]. Against this national backdrop, Arkansas was the only Southeastern state to expand Medicaid in 2014 through a process referred to as the private option, which allowed approximately 350,000 people to gain insurance coverage in this relatively poor, rural state [8].
The need to expand utilization of genetic testing for HCS is clear, especially in the group of mutation carriers who are currently unaffected by cancer and therefore have the greatest potential to benefit from risk reduction. Data from the 2015 National Health Interview Survey (NHIS) indicate that around 2.5 million people have ever received cancer genetic testing, representing roughly 1% of the population [9]. Several groups were noted to have lower rates of genetic testing, including men, Hispanics, the uninsured, and those with less education [9]. Other data document that while rates of testing have increased modestly over the last decade, the increase has occurred mainly in commercially or publicly insured women with a family history of cancer [10]. Among women with a family history of breast or ovarian cancer, 4.2% of those with commercial insurance received genetic testing in 2015, up from 2.3% in 2010 [10]. Likewise, 2.8% of women with public insurance and a family history of breast or ovarian cancer received genetic testing in 2015, an increase from 0.3% in 2010 [10]. Others have observed considerable variation in testing by race and geographic location [11].
This study sought to describe the trends in genetic testing in a state with historically low levels of cancer genetic testing. The first objective was to assess the quarterly trends of genetic testing in four categories: HBOC testing, Lynch syndrome testing, tier 2 molecular pathology procedures (billing codes which include genes such as TP53, VHL, and MLH1 based on the complexity of the testing procedure), and a composite measure of receipt of any cancer genetic testing, stratified by those with commercial, Medicaid, state employee, or Medicare coverage in Arkansas. The second objective was to identify demographic, clinical, and insurance coverage factors associated with enrollees receiving those four types of genetic testing.

Data
The Arkansas All-Payer Claims Database (APCD) is a state-level claims database that is harmonized across multiple payers and provides a nearly universal portrait of healthcare utilization of insured people in the state of Arkansas. It includes claims from commercial, Medicaid, state employee, and Medicare health plans for Arkansans. Data from January 2013 through June 2018 were used for commercial and Medicaid enrollees; due to lags in data availability, data from January 2013 through December 2017 were used for state employees and from January 2013 through December 2016 for Medicare enrollees. Medicare is the federal health insurance program for people who are 65 or older and younger persons with disabilities or end stage renal disease. Medicaid is a public insurance program for low-income families that is administered by states and jointly funded by the federal and state governments.

Study sample
A separate sample set was constructed for each of the two objectives. For the trend analysis, enrollees with at least one month of enrollment in both medical and pharmacy benefits in each quarter were identified for each of the four insurance plan types. Enrollees were excluded from the sample if they had missing age or sex information. Enrollees who had overlapping enrollment in more than one plan type were only included in one plan using the following hierarchy: Medicare > Medicaid > state employee > commercial. For example, if an individual had overlapping enrollment in Medicare and Medicaid, they were only considered enrolled in Medicare. The total number of unique enrollees in each quarter for each plan type were retained as the denominator of persons who could have received genetic testing.
For the second objective, where exploratory models were estimated, individuals with 24 months of continuous enrollment in both medical and pharmacy benefits were identified for each of the four plan types. For individuals with more than 24 months of continuous enrollment, the most recent 24 months were considered. Individuals with more than one month of overlapping enrollment in two or more plan types were included in only one plan using the following hierarchy: state employee > commercial > Medicare > Medicaid. This hierarchy was adopted based on the empirical results for the rates of healthcare claims in the overlapping segments of the respective plans. The samples in the four plan types were combined into an overall sample. Individuals with missing age or sex were excluded. Enrollees who had a cancer diagnosis prior to genetic testing in the 24-month window and those less than 10 years of age at the start of the window were also  Figure 1). For example, an individual was considered to have received HBOC testing if they underwent testing prior to a cancer diagnosis in the 24-month study period. To avoid model overfitting, the overall sample was randomly divided into training and test sets in a 1:1 ratio. The models were developed using the training sets and evaluated using the test sets.

Study measures
Genetic tests were identified from the inpatient and outpatient claims using Current Procedural Terminology (CPT-4) codes ( For the second objective, separate binary measures were created for whether a subject received testing in any of the four genetic test categories and were used as the dependent variables in separate exploratory models. The factors explored were: age (calculated at the beginning of the 24-month enrollment segment), age squared, sex (recorded on the member enrollment file), mental , and a measure of comorbidity (modified Elixhauser index). Age and age squared were included to account for a potential non-linear relationship between age and testing. Anxiety disorders, developmental disorders and other miscellaneous mental disorders were identified using ICD-9-CM and ICD-10-CM diagnosis codes. Nicotine dependence was identified using diagnosis codes or prescription claims for nicotine replacement therapy (Supplementary Table 2). The Elixhauser index was constructed using 31 comorbid conditions based on a published algorithm [12]. The existing Elixhauser index includes non-metastatic solid tumor and metastatic cancer conditions; these were removed for this study as the focus was enrollees who had genetic testing prior to a cancer diagnosis.

Statistical analyses
Scatterplots were constructed to visualize the quarterly trends. Time series models were fit to estimate the overall trends using ordinary least squares (OLS) regressions with a first-order autoregressive error term. Linear and quadratic terms were used for the time (in quarters) variable. If the quadratic terms did not attain statistical significance, the models were re-estimated using only the linear term. For the exploratory models, logistic regression was used. Due to low test rates, Firth's bias correction was applied. The predictive ability of the models were evaluated using c-statistic and Brier score for discrimination and calibration respectively. All analyses were conducted using SAS 9.4 and R 4.0.1. A twosided p-value of 0.05 was used to determine statistical significance.

Results
A total of 1,516,850 Medicaid, 1,501,828 commercial, 154,529 state employee, and 519,955 Medicare plan enrollees had at least one month of medical and pharmacy benefit enrollment in the aim 1 sample. The overall sample of unduplicated enrollees for the trend analysis was 2,520,575. The average age was 37 years; 53% were female (data not shown). For the second objective (exploratory models), a total of 1,495,960 individuals with 24 months of continuous medical and pharmacy enrollment were identified. The average age was 45 years; 56% were female (Table 2). Figure 1 shows the quarterly trends of genetic testing for each of the four genetic testing categories for each of the four health insurance plan types. Over the vast majority of quarters, rates of HBOC testing were highest for state employee enrollees, followed by commercial, Medicare, and Medicaid enrollees. Lynch syndrome testing was the highest for state employee enrollees, followed by Medicare, commercial, and Medicaid enrollees. However, rates of tier 2 molecular pathology procedures were highest for Medicare, followed by state employee, commercial, and Medicaid enrollees. Rates for any cancer genetic testing (including HBOC, Lynch syndrome, other HCS panel testing, and tier 2 molecular pathology procedures) resembled those for tier 2 molecular pathology procedures. HBOC, tier 2 molecular pathology procedures, and any cancer genetic testing increased linearly over time in Medicaid, commercial and state employee plans, as indicated by the significant positive linear trend terms in the time series models (p < 0.05; Table 3). The average quarterly increases in genetic testing for HBOC per 1 million person-quarters were greatest for state employee enrollees (16.2), followed by commercial (7.  When analyzed by sex and age group, overall HBOC testing rates for male enrollees was lower than for female enrollees, with the highest rates among female enrollees aged 18-64 years (Fig. 2). Overall testing rates for Lynch syndrome were lower among men as well, with the highest rates seen in women >65 and 18-64 years old (Fig. 2).

Trends in genetic testing
Among women, Lynch syndrome testing was much lower across age groups compared to HBOC testing, while rates for HBOC and Lynch syndrome were comparable for men. Because of the low rates of HBOC and Lynch syndrome testing among enrollees under age 18, time series models were not estimated. Testing for HBOC increased linearly over time for both men and women in the18-64-year-old age group, but not for either sex over age 64 (Table 4; Supplementary Figures 18-21). Lynch syndrome testing increased significantly over time for men aged 18-64 years and women ≥ 65 years (Table 4; Supplementary Figures 22-25). Tier 2 molecular pathology procedures and any cancer genetic testing increased linearly for male enrollees ≥65 years, with an average rate of increase of 66.8 per 1 million person-quarters but did not increase or decrease significantly for other groups (Table 4; Supplementary Figures 26-37).

Factors associated with genetic testing
The exploratory models all had moderate to high levels of discrimination with c-statistics ≥ 0.79 (Table 5). Increasing age was associated with a higher likelihood of receiving all of the genetic testing examined. Female enrollees were much more likely to receive HBOC genetic testing than male enrollees (OR=18.91; 95% CI: 13.01-28.86) and were nearly twice as likely to receive testing for Lynch syndrome (OR=1.93; 95% CI: 1.11-3.51); however, women were slightly less likely to receive a tier 2 molecular pathology procedures genetic test (OR=0.90; 95% CI: 0.83-0.99) than men. Those with a higher comorbidity burden were more likely to receive all of the genetic tests examined. Relative to those with commercial health coverage, those with state employee coverage were more likely to receive all of the genetic tests examined, while those with Medicaid were less likely to receive HBOC, Lynch Syndrome, or any cancer genetic testing. Those with Medicare coverage also were less likely to receive HBOC or Lynch syndrome testing but were more likely to receive any cancer genetic test. Nicotine use and developmental disorders were not associated with receiving any of the genetic testing examined, while having an anxiety or other type of mental disorder were associated with a higher likelihood of receiving all of the genetic tests examined. The model results revealed significant geographic variation in receiving genetic testing. Relative to the Northwest part of Arkansas, receipt of any of the genetic tests examined was generally lower across all other regions except for receiving tier 2 molecular pathology procedures, which was higher for those living in the North-Central region of the state.

Discussion
This analysis of APCD data shows that genetic testing for hereditary cancer susceptibility increased moderately in a linear fashion from 2013 through 2018 in Arkansas. Applying the time series coefficients to our baseline rates of testing suggests that testing for HBOC and Lynch syndrome nearly doubled for those with commercial and state employee coverage. In comparison, Knerr [14]. However, the absolute rates of testing for HCS in Arkansas remained low. Less than 1 out of every 10,000 persons received Lynch syndrome testing, while fewer than 5 out of every 10,000 received HBOC testing. Estimates for the prevalence of mutations in the general US population are 1/300 for Lynch syndrome and 1/400 for BRCA1/2, emphasizing that many mutation carriers remain undiagnosed [1][2][3]. Additionally, our data suggest that health plan coverage is an important determinant in accessing cancer genetic testing. State employees had the highest rates of HBOC and Lynch syndrome testing and equally high rates as Medicare enrollees for any cancer genetic testing. Medicare enrollees had the highest rate for tier 2 molecular pathology procedures. The testing rates were substantially lower for Medicaid enrollees compared to enrollees in commercial, state employee, and Medicare plans. The overall rates of HBOC and Lynch syndrome testing were highest among women 18-64 years old, while rates of tier 2 molecular pathology procedures and any cancer genetic testing were highest among men 18-64 years old. In multivariate exploratory models that controlled for key demographic differences, those with state employee coverage were 1.6 to 4.5 times more likely to receive genetic testing compared to commercial enrollees, while commercial enrollees were substantially more likely to receive any type of genetic testing than Medicaid enrollees. Type of coverage played a similarly important role in a study more narrowly focused on women receiving BRCA1/2 testing in Massachusetts; testing among privately insured women increased from 9.3 in 2011 to 18.4 in 2015, while among Medicaid enrollees, it increased from 3.7 in 2011 to 14.7 in 2015 [14].
The lower rates of genetic testing in Medicaid enrollees may be due to several factors. First, 51% of all Arkansas Medicaid recipients in 2017 were age 20 or younger, the age group that is the least likely to have cancer genetic testing [15]. However, multivariate models controlling for age found that Medicaid recipients remained the least likely to receive any of the genetic testing examined, which suggests that substantial disparities may exist in the diagnosis of hereditary cancers across economic classes. The Affordable Care Act (ACA) implemented in 2014 mandated that genetic testing for HBOC be considered a preventive service in qualified individuals and be covered without cost sharing [15]. Arkansas participated in ACA expansion of access to insurance, unlike many other Southern states; however, this participation did not yield increases in receipt of genetic testing for Medicaid enrollees comparable to those with state employee or commercial coverage. Many studies document that physicians are less likely to discuss and order genetic screening among racial/ethnic minorities and individuals with less than a college level education [9,[16][17][18]. When genetic testing is offered by the physician, the cost is often mentioned as a major hurdle, with 49% of persons considering out-of-pocket cost as a major deterrent [19]. Some companies providing testing have specific policies that preclude billing patients for testing if Medicaid does not cover the test, but not all providers offering testing know the details of these financial policies for each company. Our results echo other data that document only modest increases in genetic testing since the ACA was implemented, suggesting that this provision may have expanded access to these services but may not be sufficient to reach all those at risk for hereditary cancer [20].
Considerable variation exists among commercial payers regarding coverage for genetic testing, which could partially explain the low uptake of testing in our study. A 2015 study reported that 76% of the private payers had coverage policies for BRCA1/2 testing [21]. The coverage for multigene panel testing was low, with only 23% of the payers covering panel testing and only under the condition that all genes tested in the panel were medically necessary for the individuals [22,23]. One explanation provided by many commercial payers is that they deem multigene testing an experimental diagnostic or treatment approach [21,23,24]. Additionally, 70% of private payers required prior authorization for both single and multigene testing in a 2018 study [23]. High-quality studies demonstrating penetrance and effectiveness of multigene panels in a clearly defined population could persuade private payers to cover panel testing and likely increase its utilization; obtaining such data, though, is complicated by the differences in the genes included in panels offered by different companies. In the meantime, companies providing genetic testing for HCS have coped with the lack of coverage of gene panels by billing for testing for specific genes, such as BRCA1/2, that are routinely covered by insurance rather than all the genes included on the panel.
Women had 18-fold higher odds of testing for HBOC and had nearly 2-fold higher odds of testing for Lynch syndrome, which confirms a consistent finding that men are less likely to receive genetic testing than women [9]. Anxiety disorders and miscellaneous mental health disorders (which included conditions such as sleep and dissociative disorders) had positive associations with general genetic testing and HBOC testing in women. A previous study in women from BRCA1/2 mutation-negative families reported that women who had a higher perceived lifetime risk of cancer and higher worry about cancer were more likely to show an interest in genetic testing [25]. Another study, however, reported no association between distress and participation in BRCA testing [26]. Given the crosssectional approach in our study, we cannot comment on whether the positive association between anxiety and other mental health disorders and genetic testing is due to mental health diagnoses before or after genetic testing.
This study has several limitations. We could not evaluate genetic testing trends across racial and ethnic groups. Unfortunately, the claims data for commercial insurance lack information on race and ethnicity. We did not include enrollees with a personal history of cancer in the current analysis; our analysis of genetic testing trends This study documented a modest increase in genetic testing for HBOC and Lynch syndrome across Medicaid, commercial, state employee and Medicare plans in Arkansas, although rates were lower than that observed in other states. The exploratory models highlight the influence of sex and health care coverage as a key determinant in accessing genetic screening. Further studies are ongoing to understand the barriers to genetic testing in Arkansas.  Actual and predicted rates Tier 2 molecular pathology procedures* in female enrollees <18 years old. Supplementary Figure 30. Actual and predicted rates of Tier 2 molecular pathology procedures* in women 18-64 years old. Supplementary Figure 31. Actual and predicted rates of Tier 2 molecular pathology procedures* in women ≥65 years old. Supplementary  Figure 32. Actual and predicted rates of any cancer genetic testing* in male enrollees <18 years old. Supplementary Figure 33. Actual and predicted rates of any cancer genetic testing* in men 18-64 years old. Supplementary  Figure 34. Actual and predicted rates of any cancer genetic testing* in men ≥65 years old. Supplementary Figure 35. Actual and predicted rates of any cancer genetic testing* in female enrollees <18 years old. Supplementary  Figure 36. Actual and predicted rates of any cancer genetic testing* in women 18-64 years old. Supplementary Figure 37. Actual and predicted rates of any cancer genetic testing* in women.